K-AmpDetect 1T and K-AmpDetect 2T

What is K-AmpDetect?

K-AmpDetect 1T is a universal lateral flow dipstick for the detection of genomic amplification labeled with fluorescein isothiocyanate (FITC) and biotin.

K-AmpDetect 2T is a universal lateral flow dipstick for detection of genomic amplification labeled with fluorescein isothiocyanate (FITC), biotin, and digoxin.

The amplification products can be visualized easily by K-AmpDetect, which is more advantageous than the other detection methods because of its rapidity, low cost, ease of use, and long-term stability.

K-AmpDetect 1T
K-AmpDetect 2T

K-AmpDetect is composed of four parts: the sample pad, conjugate pad, nitrocellulose membrane, and the absorbent pad.

The sample pad is pretreated with buffer, providing a suitable pH and ion strength for detection.

The conjugate pad is sprayed with colloidal gold conjugated mouse anti-FITC.

The nitrocellulose membrane of K-AmpDetect T1 and T2 contains one test line and two test lines, respectively. For the single test line, streptavidin was sprayed on the nitrocellulose membrane to create the test line. For the two test lines, anti-digoxin (anti-DIG; Test line 1) and streptavidin (Test line 2) were sprayed on the nitrocellulose membrane. Anti-mouse antibody was sprayed on the membrane to form a control line.

K-AmpDetect 1T and K-AmpDetect 2T

Application of the K-AmpDetect

  1. Detection of conventional PCR products
  2. Detection of LAMP assay
  3. Detection of RPA products

Test Procedure

K-AmpDetect 1T
K-AmpDetect 2T
K-AmpDetect 1T and K-AmpDetect 2T

How to interpret the results of K-AmpDetect?

  1. Positive result
    The presence of two/three color band lines (C&T for K-AmpDetect 1T (left) or C & T1,T2 for K-AmpDetect 2T (right)) indicates a positive result.
  1. Negative result
    The presence of one color band line (C) indicates a negative result.
  1. Invalid result
    If the control line (C) fails to appear, the result is considered invalid.
K-AmpDetect 1T and K-AmpDetect 2T

K.Bioscience’s products have been cited by several related studies:


Rapid and simultaneous detection of Campylobacter spp. and Salmonella spp. in chicken samples by duplex loop-mediated isothermal amplification coupled with a lateral flow biosensor assay

Authors:

Sridapan, Thanawat et al.

Journal:

PLOS ONE 2021

Cited Product:

K-AmpDetect 2T

Application:

d-LAMP-LFB assay


Rapid detection of methicillin-resistant Staphylococcus aureus in positive blood cultures by recombinase polymerase amplification combined with lateral flow strip

Authors:

Srisrattakarn, Arpasiri et al.

Journal:

PLOS ONE 2022

Cited Product:

K-AmpDetect 2T

Application:

RPA-LF test


A rapid colorimetric lateral flow test strip for detection of live Salmonella Enteritidis using whole phage as a specific binder

Authors:

Charlermroj, Ratthaphol et al.

Journal:

Frontiers in Microbiology 2022

Cited Product:

AuNPs KP-05120003,
GF33,
CN95

Application:

Lateral flow assay


Lateral flow immunoassay for naked eye detection of Mycobacterium tuberculosis

Authors:

Ariffin, Nazifah et al.

Journal:

Journal of Sensors 2020

Cited Product:

Millipore C048,
Whatman FF 60/100,
Whatman 470

Application:

Lateral flow assay


Development, analytical, and clinical evaluation of rapid immunochromatographic antigen test for SARS-CoV-2 variants detection

Authors:

Szekely, Jidapa et al.

Journal:

Diagnostics 2022

Cited Product:

KBS-Ag-01,
KBS-Ag-02

Application:

COVID-19 Antigen detection