Assay Development

Let K.Biosciences (KBS) help you develop your assay!

  • Experienced team
  • Proven background in product development
  • Lateral-flow rapid tests
  • Amplicon detection

Competitive Lateral Flow Assay

For the detection of:

  • Standard antigens that spike in buffer
  • Standard antigen spike in natural samples such as urine or serum

Identify cutoff values and evaluate real samples!

Choice of key reagents from customer

  • Antibodies
  • Antigen for capture reagent (Test line)
  • Standard antigen
  • Natural sample
  • Amount of antigen in real sample from other technique.

Choice of key materials from KBS

  • Membrane
  • Conjugate particles : Colloidal gold
  • Pretreatment conjugate pad
  • Pretreatment sample pad
  • Wick
  • Backing card
  • Other materials if needed

Project Deliverables

  • Evaluation record
  • Result of proof of principle studies for competitive lateral flow assay.
  • The cutoff of test from proof of principle studies
  • Up to 20 test kits sample for evaluation from the best combination strip

Lateral Flow Sandwich Immunoassay

Proof of principle studies for sandwich lateral-flow assay of antibody detection in serum. The product is intended to be fully proof of principle by using customer’s antigen, antibody and other necessary materials.

Choice of key reagents from customer

Antibodies:

Depends on type of antibody
Anti-human IgG, IgM or IgG4

Standard antigen:

Recombinant Antigens/Native Antigens/
Synthetic Peptides

Natural sample

Choice of assay formats :

Sandwich immunoassay
IgG, IgM or IgG4 detection

Recombinant Antigen (capture):

Anti-human IgG, IgM or IgG4 (detector)

Native Antigen (capture):

Anti-human IgG, IgM or IgG4 (detector)

Synthetic peptide (capture):

Anti-human IgG, IgM or IgG4 (detector)

Choice of key materials from KBS

  • Membrane
  • Conjugate particles: colloidal gold
  • Pretreatment conjugate pad
  • Pretreatment sample pad
  • Wick
  • Backing card
  • Other materials if needed

Project Deliverables

  • Evaluation record
  • Result of proof of principle studies
  • The cutoff of test from proof of principle. Up to 20 test kit samples to evaluate the best combination strip!